<b>FORMAZAN FORMATION IN HEPATIC MACROPHAGES AFTER NITRO BLUE TETRAZOLIUM PERFUSION INTO RAT </b><b>LIVER </b>
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چکیده
منابع مشابه
Cardiovascular effects produced by systemic injections of nitro blue tetrazolium in the rat.
Nitro blue tetrazolium is a powerful electron acceptor which is widely used to localize NADPH-dependent flavin-containing enzymes known as NADPH diaphorases. By accepting electrons, nitro blue tetrazolium is known to inhibit the activity of these enzymes. The present study examined the effects of intravenous nitro blue tetrazolium on arterial blood pressure and regional blood flows in urethane-...
متن کاملFailure of nitro blue tetrazolium reduction in the phagocytic vacuoles of leukocytes in chronic granulomatous disease.
The leukocytes of patients with chronic granulomatous disease (CGD) may be identified by their failure to reduce Nitro Blue Tetrazolium (NBT) during phagocytosis. This reaction, normally detected in the phagocytic vacuole, is absent or delayed in CGD monocytes and eosinophils as well as in neutrophils, even though sonicates of normal and CGD leukocytes contain equal activities of a cyanide inse...
متن کاملNitro blue tetrazolium inhibits but does not mimic hypoxic vasoconstriction in isolated rabbit lungs.
It has been suggested that hypoxic pulmonary vasoconstriction (HPV) may mainly proceed via loss of normoxic vasodilation, forwarded by tonic O2-dependent formation of nitric oxide and superoxide (23). Both agents may stimulate guanylate cyclase, the latter via conversion to hydrogen peroxide and formation of compound I with catalase. We probed this hypothesis in perfused rabbit lungs, employing...
متن کاملActions of adenosine on nitro blue tetrazolium deposition and surface pH during intestinal reperfusion injury.
Mesenteric arteries supplying an intestinal segment were occluded for 5 minutes and then released. During reperfusion, two series of measurements were made with various substances topically applied to the extraluminal surface. In the first series, reduced nitro blue tetrazolium (NBT) was extracted from tissue and measured spectrophotometrically, as an index of oxidative damage. In the second se...
متن کاملDosimetry characterization of nitro-blue tetrazolium polyvinyl butyral films for radiation processing
Nitro-blue tetrazolium polyvinyl butyral film dosimeters (NBT-PVB) were prepared and investigated based on radiation-induced reduction of NBT. NBT-PVB film dosimeters containing different concentrations of NBT dye from 1 to 5 mM were prepared in a solution of ethanol. The dosimeters were irradiated with g-ray from Co source at doses from 5 up to 55 kGy. UV/vis spectrophotometry was used to inve...
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ژورنال
عنوان ژورنال: Biomedical Research
سال: 1988
ISSN: 0388-6107,1880-313X
DOI: 10.2220/biomedres.9.113